Therapeutic Polysaccharides


Known for their non-toxicity, biocompatibility, and biodegradability, therapeutic polysaccharides represent an important class of bio-polymers used in pharmaceutical products.

Due to its anionic charge, very high MW, high viscosity, and gelation properties, hyaluronic acid (HA) is used in many applications including the treatment of joint pains and ocular drug delivery formulations.

Stability of HA-containing pharmaceutical products is directly affected by HA concentration and MW.

Due to its very high MW and rigid structure, HA are often too large for traditional SEC.

3D Render of Polysaccharides On The Molecular Level


A Flow Injection Polymer Analysis (FIPA) method was therefore developed herein to accurately measure the bulk Mw and concentration of HA using RI and LS detectors (Table 1).

Graph of Retention Volumes of Therapeutic Polysaccharides

Figure 1. Overlay chromatogram obtained from the RI detector of HA Sample A (black) and B (green).

Table of the Molecular Weight and Concentration of Therapeutic Polysaccharides


Using FIPA coupled with RI and LS detection, rigid high molecular weight polymeric materials can be analyzed such as HA. This technique is easily used for QA/QC to monitor stability and batch-to-batch variations.

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